Improvement of the Extraction Process for High Commercial Value Pigments from Desmodesmus sp. Microalgae
نویسندگان
چکیده
Considering the high interest and commercial value of pigments from microalgae, this study aimed to stablish the best lutein and β-carotene extraction method for Desmodesmus sp., a microalgae with high biomass production. The evaluation was done considering the best solvent or solvent mixture, number of extractions, pre-treatment of dry biomass with acetone, saponification in methanol and ethanol and stability evaluation of the obtained extract. Among achieved results, the best solvent extractor was a hexane:ethanol mixture in a 1:1 (v/v) proportion. In order to obtain maximum yield, the pattern stablished includes six extractions performed for 40 minutes. Stability tests presented that xanthophylls and carotenes pigments were more stable in methanol and that xanthophylls and carotenes pigments were less negatively affected than chlorophylls in the methanolic extract. Chromatographic identification and quantification of pigments from Desmodesmus sp. microalgae revealed that its major constituents are trans-zeaxanthin, chlorophyll b, β-carotene and trans-lutein.
منابع مشابه
Carotenoids, Phenolic Compounds and Tocopherols Contribute to the Antioxidative Properties of Some Microalgae Species Grown on Industrial Wastewater.
This study aimed at investigating the potential of microalgae species grown on industrial waste water as a new source of natural antioxidants. Six microalgae from different classes, including Phaeodactylum sp. (Bacillariophyceae), Nannochloropsis sp. (Eustigmatophyceae), Chlorella sp., Dunaniella sp., and Desmodesmus sp. (Chlorophyta), were screened for their antioxidant properties using differ...
متن کاملThe effect of growth phase on the surface properties of three oleaginous microalgae (Botryococcus sp. FACGB-762, Chlorella sp. XJ-445 and Desmodesmus bijugatus XJ-231)
The effects of growth phase on the lipid content and surface properties of oleaginous microalgae Botryococcus sp. FACGB-762, Chlorella sp. XJ-445 and Desmodesmus bijugatus XJ-231 were investigated in this study. The results showed that throughout the growth phases, the lipid content of microalgae increased. The surface properties like particle size, the degree of hydrophobicity, and the total c...
متن کاملNutritional Evaluation of Australian Microalgae as Potential Human Health Supplements
This study investigated the biochemical suitability of Australian native microalgal species Scenedesmus sp., Nannochloropsis sp., Dunaliella sp., and a chlorophytic polyculture as nutritional supplements for human health. The four microalgal cultures were harvested during exponential growth, lyophilized, and analysed for proximate composition (moisture, ash, lipid, carbohydrates, and protein), ...
متن کاملIsolation, Identification and Characterization of an Electrogenic Microalgae Strain
Extracellular electron transfer involving microbes is important as it closely reflects the ability of cells to communicate with the environment. However, there are few reports on electron transfer mechanisms of pure microalgae and a lack of any model alga to study the transfer processes. In the present study, nine green microalgae species were isolated from wastewater and characterized in terms...
متن کاملHigh Protein- and High Lipid-Producing Microalgae from Northern Australia as Potential Feedstock for Animal Feed and Biodiesel
Microalgal biomass can be used for biodiesel, feed, and food production. Collection and identification of local microalgal strains in the Northern Territory, Australia was conducted to identify strains with high protein and lipid contents as potential feedstock for animal feed and biodiesel production, respectively. A total of 36 strains were isolated from 13 samples collected from a variety of...
متن کامل